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In the research sector, in vitro cultures represent one of the fundamental techniques, allowing the controlled growth of cells, tissues and microorganisms in artificial environments. The fields of application are many, ranging from biotechnology, to pharmacology to agronomy.

In order to obtain safe and reproducible results, in vitro cultures require extremely controlled environments.

 

Today we will delve into the world of in vitro cultures and see the most practical way to obtain optimal environments.

What are in vitro cultures and what are they used for

 

“In vitro” comes from Latin and means “under glass”. In this sense, in vitro culture is any technique that allows the growth of cells or tissues outside of a living organism - generally under glass - using nutritional substrates and optimal environmental conditions.

This is a very valuable technique because researchers can conduct controlled and reproducible experiments without having to resort to living organisms, thus reducing the ethical impact and optimizing the reliability of the results.

However, being outside the organisms to which they belong, in vitro cultures need extremely precise environmental conditions to thrive. This is what in vitro culture chambers are for.

Illumination Chamber

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In Vitro Culture Chambers: Features and Benefits

 

In vitro culture chambers are hermetically sealed environments, within which it is possible to alter, set, control and monitor a series of environmental parameters.

Temperature and relative humidity are the main environmental parameters to be adjusted in order to reproduce an environment suitable for the individual in vitro culture, but almost always the simulation of sunlight is also necessary.

For this reason, in vitro culture chambers have different LED lighting systems that reproduce various types of light, such as the day/night difference and different levels of intensity.

Application Sectors and International Regulations

 

In vitro culture chambers assist scientific research in many sectors.

In the biotechnology sector, for example, in vitro culture chambers are used for the development of new drugs and for cytotoxicity tests on cultured cells, ensuring high standards of safety and efficacy.

In agronomy and plant research, in vitro culture chambers support the in vitro propagation of plants, allowing the reproduction of genetically improved species or species resistant to particular environmental conditions.

We also find in vitro culture chambers in the cosmetics industry, where they are used to test the safety and efficacy of products on cells, avoiding the need for animal testing.

At a regulatory level, the main international bodies that provide guidelines for the various in vitro tests are the OECD, with its OECD Test Guidelines, and the ICH, in various standards such as the ICH S3A/S3B and the ICH M7.

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FDM In Vitro Culture Chambers

 

For over 70 years, FDM – Environment Makers has been offering cutting-edge solutions for environmental simulation.

Our illumination chambers are the ideal tool for in vitro culture research.

Thanks to precise temperature and relative humidity control and a lighting set capable of reproducing multiple light qualities, our in vitro culture chambers guarantee ideal conditions for any type of research, ensuring reproducibility, reliability and sustainability in scientific processes.

For further doubts and questions, please do not hesitate to contact us.

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